Efficient Heritable Gene Expression Readily Evolves in RNA Pools
نویسندگان
چکیده
منابع مشابه
Efficient and heritable gene targeting in tilapia by CRISPR/Cas9.
Studies of gene function in non-model animals have been limited by the approaches available for eliminating gene function. The CRISPR/Cas9 ( C: lustered R: egularly I: nterspaced S: hort P: alindromic R: epeats/ C: RISPR AS: sociated) system has recently become a powerful tool for targeted genome editing. Here, we report the use of the CRISPR/Cas9 system to disrupt selected genes, including nan...
متن کاملEpigenetic Regulation by Heritable RNA
Genomic concepts are based on the assumption that phenotypes arise from the expression of genetic variants. However, the presence of non-Mendelian inheritance patterns provides a direct challenge to this view and suggests an important role for alternative mechanisms of gene regulation and inheritance. Over the past few years, a highly complex and diverse network of noncoding RNAs has been disco...
متن کاملGene expression plasticity evolves in response to colonization of freshwater lakes in threespine stickleback.
Phenotypic plasticity is predicted to facilitate individual survival and/or evolve in response to novel environments. Plasticity that facilitates survival should both permit colonization and act as a buffer against further evolution, with contemporary and derived forms predicted to be similarly plastic for a suite of traits. On the other hand, given the importance of plasticity in maintaining i...
متن کاملGene expression evolves faster in narrowly than in broadly expressed mammalian genes.
Despite much recent interest, it remains unclear what determines the rate of evolution of gene expression. To study this issue we develop a new measure, called "Expression Conservation Index" (ECI), to quantify the degree of tissue-expression conservation between two homologous genes. Applying this measure to a large set of gene expression data from human and mouse, we show that tissue expressi...
متن کاملTRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Molecular Evolution
سال: 2017
ISSN: 0022-2844,1432-1432
DOI: 10.1007/s00239-017-9800-1